FIGURE

Fig. 1

ID

ZDB-FIG-210217-49

Publication

Begeman et al., 2020 - Decoding an Organ Regeneration Switch by Dissecting Cardiac Regeneration Enhancers

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Fig. 1

Regeneration-dependent activity of cardiac LEN (cLEN). (A) Schematic of lepb regeneration enhancer (LEN) containing two tissue-specific regeneration modules. A transgene construct examined for cLEN activity consists of 317 bp of cLEN, lepb 2 kb upstream promoter (P2) and EGFP reporter sequences. (B) Schematic of cLEN annotated with predicted TF binding sites. (C-F) Zebrafish strain and experimental strategy undertaken to ablate CMs (C) and images of uninjured and ablated hearts in larvae (D), juveniles (E) and adults (F). (G-I) Dynamic cLEN activity during zebrafish heart regeneration. (G) Experimental design to determine cLEN activity during heart regeneration. (H) Quantification of EGFP expression intensity normalized to images of wild-type uninjured hearts at the wound area. The data are presented as the mean±s.d. Numbers of animals are shown in Table S3. *P<0.05; **P<0.01; N.S., not significant. One-way ANOVA with Tukey's post-hoc test. (I) Images of cardiac sections of uninjured and regenerating hearts. Scale bars: 100 µm in E″,F″,I; 50 µm in D′.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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