Figure 5
- ID
- ZDB-FIG-201223-17
- Publication
- Chin et al., 2020 - Cohesin mutations are synthetic lethal with stimulation of WNT signaling
- Other Figures
-
- Figure 1.
- Figure 2
- Figure 2—figure supplement 1.
- Figure 2—figure supplement 2.
- Figure 3
- Figure 3—figure supplement 1.
- Figure 3—figure supplement 2.
- Figure 3—figure supplement 3.
- Figure 4
- Figure 4—figure supplement 1.
- Figure 4—figure supplement 2.
- Figure 5
- Figure 5—figure supplement 1.
- Figure 5—figure supplement 2.
- Figure 5—figure supplement 3.
- Figure 6
- Figure 6—figure supplement 1.
- Figure 6—figure supplement 2.
- Figure 7.
- All Figure Page
- Back to All Figure Page
(A) Immunoblot of the membrane fraction of parental (WT) and cohesin-deficient MCF10A cells shows increased basal level of β-catenin phosphorylation at Ser33/37/Thr41. (B) Immunoblot of the cytoplasmic fraction shows increased level of both total and phosphorylated β-catenin at Ser675 after parental (WT) and cohesin-deficient MCF10A cells were treated with LY2090314 at 100 nM for 24 hr. (C) Quantification of protein levels for total and phosphorylated β-catenin at Ser675. n = 3 independent experiments, mean ± s.d., one-way ANOVA: *p≤0.05, **p≤0.01. (D) Immunofluorescence images show cytosolic accumulation of active β-catenin in cohesin-deficient MCF10A cells treated with LY2090314 100 nM for 24 hr, relative to parental (WT) MCF10A cells. White arrows indicate puncta of β-catenin (pSer675). Scale bar = 25 μM. Full length blots and molecular size markers are available for A,B in Figure 5—source data 1. Source quantification data is available for C in Figure 5—source data 2.
|