Hox genes are involved in gsx2 expression and IO neuronal development. (A-D) 3 dpf control morphant (A,B, n=5) and pbx2/4 morphant (C,D, n=4) mafbaGFF;UAS;GFP;gsx2:RFP larvae were stained using anti-RFP (magenta) and anti-GFP (green) antibodies. (E-H) 5 dpf control morphant (E,F, n=4) and pbx2/4 morphant (G,H, n=4) 28C;UAS:GFP larvae stained using anti-Pvalb7 (magenta) and anti-GFP (green) antibodies. (I,J) Expression of pou4f1 in 5 dpf control morphant (I) and pbx2/4 morphant (J) larvae. The pou4f1 signal is marked by arrowheads. (K,L) Length of the gsx2:RFP+ hindbrain region (K) and number of 28C;UAS:GFP+ IO neurons (L) in control and pbx2/4 morphant larvae. (M-P) 3 dpf control (M,N, n=4) and 25 pg hoxb4a RNA-injected (O,P, n=4) larvae were stained with anti-RFP (magenta) and anti-GFP (green) antibodies. (Q,R) Length of the gsx2:RFP+ hindbrain region (Q) and number of 28C;UAS:GFP+ neurons (R) in control and hoxb4a RNA-injected larvae. (S,T) Length of the gsx2:RFP+ hindbrain region in control (n=5), aldh1a2 morphant (n=8) and hoxb4a RNA-injected aldh1a2 morphant larvae (n=8) (S); in control (1% DMSO-treated, n=4), DEAB-treated (n=5), hoxb4a RNA-injected and DEAB-treated larvae (n=5) (T). (U,V) Number of 28C;UAS:GFP+ neurons in control (n=4), aldh1a2 morphant (n=4), and hoxb4a RNA-injected aldh1a2 morphant larvae (n=4) (U); in control (1% DMSO-treated, n=5), DEAB-treated (n=5), hoxb4a RNA-injected and DEAB-treated larvae (n=5) (V). *P<0.05; **P<0.01; ***P<0.001 (Student's t-test for K,L,Q,R, and one-way ANOVA with Tukey's multiple comparison test for S-V). Data are mean±s.e.m. with individual values indicated. Scale bars: 100 µm in A (applies to A-D), M (applies to M-P) and I (applies to I,J); 50 µm in E (applies to E,G); 20 μm in F (applies to F,H).
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