FIGURE

Figure 5

ID

ZDB-FIG-201003-33

Publication

Converse et al., 2020 - The zinc transporter ZIP9 (Slc39a9) regulates zinc dynamics essential to egg activation in zebrafish

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Figure 5

Zinc is stored in cortically-localized vesicles that undergo exocytosis upon egg activation. (A, B)zip9^+/+(A) and zip9^-/-(B) eggs show intracellular zinc staining (arrows, 0 min) in cortically-localized vesicles that are reduced in number at 2.5–10 min post-activation. (C, D) Extracellular zinc is observed 2.5–10 min post-activation of zip9^+/+(C) and zip9^-/-(D) eggs. (E) Time trace of normalized extracellular zinc fluorescence by ROI analysis (ROI denoted by yellow circle in C, 0 min) 0–10 min post-activation of zip9^+/+ and zip9^-/- eggs. Visualization was performed on eggs from a minimum of 3 females/strain. For (E), data represents means ± SEM; n = 5–6. Significance was determined by Welch's t-test (*, P < 0.05; **, P < 0.01).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	slc39a9^{utz1Tg/utz1Tg}
Observed In:	unfertilized egg zinc ion transmembrane transport
Stage:	Adult

Phenotype Detail

Acknowledgments

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