ZFIN Image: Converse et al., 2020, Figure 5

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Figure Caption

Figure 5

Zinc is stored in cortically-localized vesicles that undergo exocytosis upon egg activation. (A, B)zip9^+/+(A) and zip9^-/-(B) eggs show intracellular zinc staining (arrows, 0 min) in cortically-localized vesicles that are reduced in number at 2.5–10 min post-activation. (C, D) Extracellular zinc is observed 2.5–10 min post-activation of zip9^+/+(C) and zip9^-/-(D) eggs. (E) Time trace of normalized extracellular zinc fluorescence by ROI analysis (ROI denoted by yellow circle in C, 0 min) 0–10 min post-activation of zip9^+/+ and zip9^-/- eggs. Visualization was performed on eggs from a minimum of 3 females/strain. For (E), data represents means ± SEM; n = 5–6. Significance was determined by Welch's t-test (*, P < 0.05; **, P < 0.01).

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Phenotype details

Acknowledgments

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