Figure 7—figure supplement 2.

(A) Schematic to ease the interpretation of the adjacent rank-rank hypergeometric overlap (RRHO) plots allowing to compare the overlaps in gene expression changes induced by mondoa knockout (KO, MZmondoa) or mondoa knockdown (KD). (B) RRHO plot comparing differential gene expression of mondoa-mo vs. mondoa-mis injected (y-axis) embryos and MZmondoa mutants with unaffected epiboly vs. wild-type embryos (x-axis). (C) RRHO plot comparing differential gene expression of mondoa-mo vs. mondoa-mis injected (y-axis) embryos and MZmondoa mutants with affected epiboly phenotype vs. wild-type embryos (x-axis). (D) RRHO plot comparing differential gene expression of mondoa-mo vs. mondoa-mis injected embryos with a randomly ordered gene list. (E) Wald statistic of mondoa-mo vs. mondoa-mis injected embryos (x-axis) and unaffected MZmondoa mutants vs. wild-type (WT) embryos (y-axis). Genes with a value >5 are represented by a triangle. (F, G) Expression of nsdhl (F) and cyp11a1 (G) in mondoa-mo and mondoa-mis injected embryos and in MZmondoa mutants affected or unaffected in epiboly and wild-type control embryos. nsdhl expression is compensated in MZmondoa mutants. cyp11a1 expression is upregulated in both mutants, reaching higher levels in mutants with affected epiboly.