FIGURE 4

Myocardial tbx20 overexpression affects expression of various injury responsive genes following heart injury. (A) Schematic of DOX treatment, heart injury and tissue collection procedures. RNA samples were extracted from the ventricular apices (below the dashed line). For each group, 6–8 samples were collected, and three independent biological replicates were carried out. (B) Significantly enriched gene ontology (GO) analysis based on RNA-seq results revealed upregulated gene categories (upper panel), and downregulated gene categories (lower panel). (C–F) Bar graphs indicate qPCR analyses of relative expression levels of genes associated with “Cell cycle regulators” (C), “ECM function” (D), “Immune response” (E) and “Sarcomere related genes” (F) in ventricular apices from DOX-treated Tg(TRE3G:tbx20^CMOE) and Tg(TRE3G:tbx20) at 7 dpa. Data represents three biological replicates. Mean ± SEM, *p < 0.05, **p < 0.01, ***p < 0.001. (G–J) Representative images of ISH analyses for hbegfb(G,H) and mylk3(I,J) on 7 dpa heart sections from Tg(TRE3G:tbx20^CMOE) and Tg(TRE3G:tbx20) zebrafish with DOX treatment. Scale bar: 100 μm.