Fig. 6

Conserved developmental states between embryonic and postembryonic retinogenesis. (A) Visualization of distinct clusters of 14-dpf CMZ progenitor cells using t-SNE. Cells are colored by their cluster assignments. Clusters are indicated by their corresponding labels. (B) The pseudo-time trajectories of distinct clusters of 14-dpf CMZ progenitor cells. Cells are colored by their cluster assignments. (C) The expressions of cluster-specific marker genes across pseudo-time. Genes are colored by their cluster assignments. (D) In situ hybridization images of Clusters 1- to 4-specific marker genes (fabp11a, her4.2, atoh7 and vsx1) in the CMZ of the retinas at 14 dpf. Genes are colored by their corresponding clusters. The CMZ boundaries are indicated by the white dashed lines. The areas showing in situ signals (green) are indicated by the region between yellow dashed lines. The nuclei are labeled by DAPI (magenta). Scale bar: 30 μm. (E) Schematic showing the spatial distribution of cells of Clusters 1 to 4 in the CMZ of the retina at 14 dpf. (F) Hierarchal clustering of distinct clusters from embryonic (48-hpf RPCs) and postembryonic (14-dpf CMZ progenitor cells) stages (Jaccard distance metric, average linkage). Yellow arrow indicates the cluster with specific expression of mafba. (G) In situ hybridization images of mafba in 48-hpf RPCs and the CMZ progenitor cells of 14-dpf retina. The 48-hpf retina and the CMZ of 14-dpf retina are indicated by the white dashed lines. Yellow arrowheads indicate the areas showing in situ signals (green). Scale bars: 30 μm.