FIGURE

Fig 4

ID

ZDB-FIG-200610-25

Publication

Santos-Ledo et al., 2020 - Alternative splicing of jnk1a in zebrafish determines first heart field ventricular cardiomyocyte numbers through modulation of hand2 expression

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Fig 4

<italic>jnk1a/b</italic> mutants.

Sequences of genomic DNA for jnk1a(A) and jnk1b(B) showing targeting sequence for CRISPR-Cas9 guide RNA; deletions produced; translated peptide sequence (* = stop); and restriction enzymes that identify mutants. Both jnk1a and jnk1b mutations disrupt sequence within exon 6 and predicted to lead to loss of all alternatively spliced transcripts by nonsense-mediated decay. (C) Maternal zygotic (MZ) jnk1a, MZjnk1b and MZjnk1a/ MZjnk1b mutants all appear grossly normal and are fertile. (D) RT-PCR using primers specific for jnk1a and jnk1b exon 7 and 8 show nonsense-mediated decay in MZjnk1a and MZjnk1b mutants. * = p<0.05, ** = p<0.01, **** = p<0.0001. n = 3 (3 separate clutches of 40 embryos obtained from different pairs of fish). Scale bar 1mm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	mapk8a^n2/n2 (AB) mapk8b^n3/n3 (AB) mapk8a^n2/n2; mapk8b^n3/n3 (AB)
Observed In:	whole organism
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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