FIGURE

Fig. 7

ID

ZDB-FIG-200523-27

Publication

Saito et al., 2010 - Identification of zebrafish Fxyd11a protein that is highly expressed in ion-transporting epithelium of the gill and skin and its possible role in ion homeostasis

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Fig. 7

Effects of zFxyd11 knockdown. (A–C) Immunofluorescence staining was performed on zfxyd11-MO-injected (A), control-MO-injected (B), and zfxyd11-MO with zfxyd11a mRNA-injected larvae (C) with anti-zFxyd11 antiserum (green), along with anti–Na⁺–K⁺-ATPase antiserum (red). No signal for zFxyd11 was detected in zfxyd11 morphants (A). (D) The number of Na⁺–K⁺-ATPase–positive cells in the skin was counted and is represented as the mean ± SEM. Significant differences were evaluated by one-way ANOVA, Tukey's multiple comparison test (***p < 0.001, n = 7). (E) Immunofluorescence staining was performed on zfxyd11-MO-injected (left panel), control-MO-injected larvae (right panel) with anti–vH⁺-ATPase antiserum. (F) The number of vH⁺-ATPase–positive cells in the skin was counted and is represented as the mean ± SEM. The number of vH⁺-ATPase-positive cells was not affected by zFxyd11 knockdown (n = 7).

Expression Data

Gene:	fxyd11
Antibodies:	Ab1-fxyd11 Ab2-atp6v1b2 Ab6-atp1a1
Fish:	WT WT + MO1-fxyd11
Knockdown Reagent:	MO1-fxyd11
Anatomical Terms:	NaK ionocyte vH ionocyte
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-fxyd11
Knockdown Reagent:	MO1-fxyd11
Observed In:	NaK ionocyte vH ionocyte
Stage Range:	High-pec to Long-pec

Phenotype Detail

Acknowledgments

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