FIGURE

Figure 4

ID

ZDB-FIG-200430-3

Publication

Park et al., 2020 - Zebrafish B cell acute lymphoblastic leukemia: new findings in an old model

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Figure 4

Gene expression in individual <italic>hMYC</italic> B- and T-ALL cells.

(A) rag2:hMYC;lck: GFP fish with dim (left) and bright (center) cancers. B-ALL is only visible with 1.5 s exposure (upper left); T-ALL is visible with either 1.5 s (upper middle) or 200 ms (lower middle) exposures. Control lck:GFP fish images are shown at right, where only the thymus is visible. (B) Flow cytometry plots of the same ALL samples demonstrate GFP^lo B-ALL and GFP^hi T-ALL specimens. (C) Gene expression in single B- and T-ALL cells of the same cancers (n = 10 cells for each), as determined by Fluidigm™ Biomark qRT-PCR. B-ALL cells express B cell, but not T cell, genes; T-ALL show the opposite pattern. Expression of eef1a1l1 (homologue of human eukaryotic translation elongation factor 1 alpha 1, aka EF1A) was used as a threshold control for the presence of RNA in each well. CT values were converted to Log2Ex for visualization (scale bar at left).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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