FIGURE

Fig 7

ID

ZDB-FIG-200429-13

Publication

Arribat et al., 2020 - Spastin mutations impair coordination between lipid droplet dispersion and reticulum

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Fig 7

Spastin influences transcription and location of HSP-related proteins to control ER/LD dynamics.

(A) Quantification of REEP1, Atlastin1, Spartin and Seipin transcript by qPCR in 8 months old zebrafish brain. n = 4 animals per group. (B) Quantification of REEP1, Atlastin1, Spartin and Seipin transcript by qPCR in 8 months old zebrafish skeletal muscle. n = 4 animals per group. (C-D) Confocal microscopy images of HeLa cells overexpressing human Seipin with Spastin M1 isoforms (wild-type and mutants) treated with oleic acid (OA) for 18h. Cherry-tagged Spastin appears in red, Seipin in green, LDs (Bodipy) in magenta and nucleus (Hoechst) in blue. (E) Percentage of cells exhibiting tubular ER along MTs. (F-G) Confocal microscopy images of HeLa cells overexpressing human REEP1 with Spastin M1 isoforms in basal conditions (F) or after 18h administration of OA (G). Cherry-tagged Spastin appears in red, REEP1 in green, LDs (Bodipy) in magenta and nucleus (Hoechst) in blue. Bars are mean ± SEM, ^#P = 0.08, *P < 0.05, **P < 0.01, ***P<0.001 (unpaired t‐test). See also S3 Fig and S4 Fig.

Expression Data

Genes:	atl1 bscl2 reep1 spartb
Fish:	WT spast^ula1/ula1
Anatomical Terms:	brain skeletal muscle
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	spast^ula1/ula1
Observed In:	brain skeletal muscle
Stage:	Adult

Phenotype Detail

Acknowledgments

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