FIGURE

Fig. 2

ID

ZDB-FIG-200427-2

Publication

Wu et al., 2020 - Role of the ADCY9 gene in cardiac abnormalities of the Rubinstein-Taybi syndrome

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Fig. 2

Effects of adcy9 knock down on macrophage migration. a-f Representative bright field and fluorescent images of TG (zlyz: EGFP) larvae at 6 days postfertilization (dpf). Control MO injected fish show the normal distribution of labeled cells (b and c). Compared with control fish, embryos injected with adcy9-e3i3-MO present potent macrophage migration in heart (e and f, red circle area). g-k Quantification of the macrophage number at heart shows a 13.3-fold increased in adcy9 morphants. Columns, mean; bars, SEM (n = 10; Student’s t test; ***P < 0.0001;). dpf, days post fertilization. l Endogenous efnb2a, COX2, mmp9, ptp-rb and pik3r2 in control and adcy9 morphants assessed by qRT-PCR (n = 6–10 individual embryos). ns, not significant

Expression Data

Genes:	mmp9 pik3r2 ptprb
Fish:	AB AB + MO1-adcy9
Knockdown Reagent:	MO1-adcy9
Anatomical Term:	whole organism
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO1-adcy9 rj1Tg + MO1-adcy9
Knockdown Reagent:	MO1-adcy9
Observed In:	heart macrophage chemotaxis whole organism
Stage Range:	Protruding-mouth to Day 6

Phenotype Detail

Acknowledgments

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