FIGURE 6

Rab28 transgenic zebrafish have mild to moderate visual defects at 5 dpf. (A) Box and whisker plot of optokinetic response (OKR) assay of 5 dpf larvae overexpressing GFP-Rab28 WT, Q72L (GTP-preferring) or T26N (GDP-preferring). Larvae expressing the Q72L variant display greater variability and an overall reduction in OKR scores. Box extremities represent 1st and 3rd quartiles; whiskers are maximum and minimum values. Data are from three independent replicates; at least 30 larvae analyzed per strain over three experimental replicates; p-value derived from one-way ANOVA. (B,C) Representative activity traces of 5 dpf transgenic and sibling larval activity over the course of the entire VMR assay. Black and yellow bars indicate dark and light conditions, respectively. (D,E) Box and whisker plots of the OFF and ON peak activity of 5 dpf transgenic and sibling larvae. Data are from three independent replicates and are the average of 5 s of activity following light changes. At least 64 larvae were analyzed per strain. p-Value derived from unpaired t-test (D).