FIGURE

Figure 5

ID

ZDB-FIG-200325-76

Publication

Mawed et al., 2020 - Strategy of Hepatic Metabolic Defects Induced by beclin1 Heterozygosity in Adult Zebrafish

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Figure 5

Dysregulated phosphoinositide-3-kinase (PI3K), the serine-threonine protein kinase (AKT) and autophagy pathways in the liver of beclin1^+/− zebrafish. Expressions of mRNA in 16-month-old zebrafish evaluated by qRT-PCR for PI3K subunits and anti-apoptotic genes (A) and autophagy-related genes (B). (C) Representative immunoblots showing the relative protein expression of Akt, P-Akt, P62, LC3-I-II, Atg5-Atg12 conjugate. β-actin was used as vehicle control. (D) The ratio of P-Akt/Akt, P62/β-actin, LC3-II/LC3-I, and Atg5-atg12/β-actin quantified by NIH software Image J. (E) (a–c) Immunofluorescence of P62 showing protein increasing around the necrosis tissue of beclin1^+/−.(d–f) Immunofluorescence of liver tissues probed against LC3-II revealed a severe reduction in beclin1^+/−. CV: central vein; NC: Necrosis. Data expressed as mean ± SD. * p < 0.05, ** p < 0.01, and *** p < 0.001.

Expression Data

Genes:	akt1s1 atg5 atg7 atg12 bcl2a mcl1a pik3ca pik3r1 sqstm1
Antibodies:	Ab3-akt Ab4-map1lc3 Ab4-sqstm1 Ab5-akt Ab6-atg5
Fish:	AB atg7^hza54/+ (AB) atg7^hza55/+ (AB) becn1^hza56/+ (AB)
Anatomical Term:	male organism liver
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	atg7^hza54/+ (AB) atg7^hza55/+ (AB) becn1^hza56/+ (AB)
Observed In:	male organism liver
Stage:	Adult

Phenotype Detail

Acknowledgments

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