FIGURE

Fig 7

ID

ZDB-FIG-200325-64

Publication

Takemoto et al., 2020 - Sycp2 is essential for synaptonemal complex assembly, early meiotic recombination and homologous pairing in zebrafish spermatocytes

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Fig 7

RPA and γH2AX signals were severely reduced in <italic>sycp2</italic><sup><italic>-/-</italic></sup> spermatocytes.

A: Staining of sycp2^+/+ and sycp2^-/- spermatocyte chromosomal spreads with anti-human RPA, anti-Sycp1 and anti-Sycp3 antibodies. Note that the human RPA protein (NP_002936.1) shares 63.5% identity and 80.9% similarity with the zebrafish protein (NP_956105.2). In sycp2^+/+ spermatocytes, RPA appears at leptonema in faint foci, which become brighter at zygonema and disappear at pachynema. In sycp2^-/- spermatocytes, RPA foci were rarely detected; only a few foci were observed in some cells. B: Immunostaining of sycp2^+/+ (i, ii) and sycp2^-/- (iii, iv) spermatocyte chromosomal spreads with anti-γH2AX, anti-Sycp3 and Sycp1 antibodies. Individual images with each antibody and a merged image are shown. The wild-type (sycp2^+/+) nuclei are at early to mid-zygonema. Scale bars, 5 μm.

Expression Data

Genes:	sycp1 sycp3
Antibodies:	Ab1-h2afx Ab1-rpa2 Ab1-sycp1 Ab1-sycp3
Fish:	WT sycp2^{zf3287/zf3287}
Anatomical Term:	spermatocyte chromatin
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	sycp2^{zf3287/zf3287}
Observed In:	spermatocyte chromatin
Stage:	Adult

Phenotype Detail

Acknowledgments

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