IMAGE

Fig 7

ID

ZDB-IMAGE-200325-65

Genes

sycp1
sycp3

Antibodies

Source

Figures for Takemoto et al., 2020

Image

Figure Caption

Fig 7 RPA and γH2AX signals were severely reduced in <italic>sycp2</italic><sup><italic>-/-</italic></sup> spermatocytes.

A: Staining of sycp2^+/+ and sycp2^-/- spermatocyte chromosomal spreads with anti-human RPA, anti-Sycp1 and anti-Sycp3 antibodies. Note that the human RPA protein (NP_002936.1) shares 63.5% identity and 80.9% similarity with the zebrafish protein (NP_956105.2). In sycp2^+/+ spermatocytes, RPA appears at leptonema in faint foci, which become brighter at zygonema and disappear at pachynema. In sycp2^-/- spermatocytes, RPA foci were rarely detected; only a few foci were observed in some cells. B: Immunostaining of sycp2^+/+ (i, ii) and sycp2^-/- (iii, iv) spermatocyte chromosomal spreads with anti-γH2AX, anti-Sycp3 and Sycp1 antibodies. Individual images with each antibody and a merged image are shown. The wild-type (sycp2^+/+) nuclei are at early to mid-zygonema. Scale bars, 5 μm.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.