FIGURE

Fig. 4

ID

ZDB-FIG-200224-10

Publication

Brock et al., 2019 - Stem cell proliferation is induced by apoptotic bodies from dying cells during epithelial tissue maintenance

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Fig. 4

ESAB (epithelial stem cell-derived apoptotic body)-delivered Wnt8a is required for apoptosis-induced stem cell division. a–f Maximum intensity projections of confocal images for bromodeoxyuridine (BrdU) (green) and p63 (magenta) after perturbation of Wnt8a signaling (a–c), and in combination with induction of apoptosis (d–f) (scale = 50 µm). g Mean number of BrdU-positive cells from individual larvae after perturbation of Wnt8a (n = 9) and in combination with induced apoptosis (n = 10). h Quantitative analysis of ESAB production from controls (n = 35) and after treatment with apoptosis inhibitor (AI) (n = 20), the Wnt inhibitor IWR (n = 10) or overexpression of Wnt8a (n = 13). Data are represented as mean ± sd; ****p < 0.0001. One-way analysis of variance (ANOVA) with Dunnett’s mutiple comparisons test (g, h)

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	c264Tg; utm2Tg; zc1036aEt c264Tg; utm2Tg; w34Tg; zc1036aEt c264Tg; utm2Tg; zc1036aEt + CRISPR5-wnt8a + CRISPR6-wnt8a
Conditions:	chemical ablation: epidermal stem cell, chemical treatment by environment: apoptosis inhibitor chemical ablation: epidermal stem cell, chemical treatment by environment: metronidazole chemical ablation: epidermal stem cell, chemical treatment by environment: metronidazole, heat shock
Knockdown Reagents:	CRISPR5-wnt8a CRISPR6-wnt8a
Observed In:	epidermal stem cell apoptotic body epidermal stem cell cell population proliferation
Stage:	Day 4

Phenotype Detail

Acknowledgments

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