FIGURE

Fig. 6.

ID

ZDB-FIG-200205-11

Publication

Clark et al., 2019 - Establishment and validation of an endoplasmic reticulum stress reporter to monitor zebrafish ATF6 activity in development and disease

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Fig. 6.

Chemical ER stressors activate reporter expression. (A) Zebrafish embryos were treated with ER stressors at 1 dpf. Quantification (dashed line) revealed that 5XATF6RE:d2GFP expression was significantly higher at 8 hpt with thapsigargin (P=0.0004) and brefeldin A (P=0.0003) treatment, at 24 hpt with tunicamycin (P=0.0001) and brefeldin A (P=0.0001) treatment, and at 48 hpt with tunicamycin (P=0.0001) and brefeldin A (P=0.0065) treatment. **P≤0.01; ***P≤0.001; ****P≤0.0001; unpaired one-way ANOVA with Dunnett's post-hoc test with respect to DMSO control group. All error bars are s.e.m. (B) Representative images showing increased 5XATF6RE:d2GFP expression in the spinal cord of embryos (at 48 hpt) treated with tunicamycin or brefeldin A compared to the DMSO-treated control. Scale bars: 1 mm (A); 0.1 mm (B).

Expression Data

Gene:	d2EGFP
Fish:	mw85Tg
Conditions:	chemical treatment by environment: brefeldin A chemical treatment by environment: 1,4-dithiothreitol chemical treatment by environment: thapsigargin chemical treatment by environment: tunicamycin
Anatomical Term:	whole organism
Stage Range:	Prim-5 to Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	mw85Tg
Conditions:	chemical treatment by environment: brefeldin A chemical treatment by environment: thapsigargin chemical treatment by environment: tunicamycin
Observed In:	whole organism
Stage Range:	Prim-5 to Protruding-mouth

Phenotype Detail

Acknowledgments

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