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ZDB-FIG-200131-27

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Fukuda et al., 2019 - Mechanical Forces Regulate Cardiomyocyte Myofilament Maturation via the VCL-SSH1-CFL Axis

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Fig. 2

Cardiac Contractility Is Required for Myofilament Maturation

(A) 3D images of 103 hpf hearts treated with or without tricaine from 72 to 103 hpf, and of 103 hpf myh6 and slc8a1a morphant hearts. myh6 morphant ventricles exhibit thinner myofilaments but a WT-like myofilament banding pattern (arrowheads). Myofilament thickness measured in 103 hpf ventricles (n = 4–5 ventricles).

(B) 3D images of 78 hpf hearts treated with DMSO or IBMX from 54 to 78 hpf. 78 hpf heart rate measurement (n = 5 larvae) and ventricular myofilament thickness (n = 4–5 ventricles). Error bars, SEM. ^∗p < 0.05 and ^∗∗p < 0.001 by ANOVA followed by Tukey’s HSD test. Scale bars, 20 μm.

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Reprinted from Developmental Cell, 51(1), Fukuda, R., Gunawan, F., Ramadass, R., Beisaw, A., Konzer, A., Mullapudi, S.T., Gentile, A., Maischein, H.M., Graumann, J., Stainier, D.Y.R., Mechanical Forces Regulate Cardiomyocyte Myofilament Maturation via the VCL-SSH1-CFL Axis, 62-77.e5, Copyright (2019) with permission from Elsevier. Full text @ Dev. Cell