FIGURE

Fig. 6

ID

ZDB-FIG-200130-12

Publication

Huning et al., 2020 - Two paralogous znf143 genes in zebrafish encode transcriptional activator proteins with similar functions but expressed at different levels during early development

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Fig. 6

Analysis of specific gene knockdown in CRISPRi experiments. Quantitative RT-PCR was used to analyze relative levels of znf143a and znf143b transcripts. The amounts of each znf143 cDNA were determined relative to those in the dCas9 injection control after normalization to the geometric mean of the widely-expressed transcripts (ef1α and rpl13α). The height of each column represents the mean of 4 or 5 independent injection experiments, and error bars represent standard deviation from the mean. A single asterisk signifies a p-value < 0.05 relative to the control sample lacking any sgRNAs, while a double asterisk signifies a p-value < 0.01

Expression Data

Genes:	znf143a znf143b
Fish:	AB/TL AB/TL + CRISPR1-znf143a + CRISPR2-znf143a + CRISPR3-znf143a AB/TL + CRISPR1-znf143b + CRISPR2-znf143b + CRISPR3-znf143b
Knockdown Reagents:	CRISPR1-znf143a CRISPR1-znf143b CRISPR2-znf143a CRISPR2-znf143b CRISPR3-znf143a CRISPR3-znf143b
Anatomical Term:	whole organism
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB/TL + CRISPR1-znf143a + CRISPR2-znf143a + CRISPR3-znf143a AB/TL + CRISPR1-znf143b + CRISPR2-znf143b + CRISPR3-znf143b
Knockdown Reagents:	CRISPR1-znf143a CRISPR1-znf143b CRISPR2-znf143a CRISPR2-znf143b CRISPR3-znf143a CRISPR3-znf143b
Observed In:	whole organism
Stage:	Prim-5

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ BMC Mol Cell Biol