Fig. 5

Loss of LRE Protein Uptake Impairs Growth and Survival in Larval Zebrafish

(A and B) Survival rates of cubn ^pd1169 +/−, −⁄− (A), and dab2 ^pd1162 +/−, −⁄−(B) 30 dpf larvae raised under different feeding conditions: non-calorie restricted standard diet (SD), calorie-restricted high-protein diet (HP) and calorie-restricted low-protein diet (LP). n = 5, 9, 6, 14, 9, and 14 (A; left to right), n = 9, 10, 9, 9, 9, and 9 (B; left to right). Data points are the survival rates of each tank subjected to the experiment.

(C) Comparison of body lengths of cubn ^pd1169 +/− (n = 29), −⁄− (n = 57), and dab2 ^pd1162 +/− (n = 69), −⁄− (n = 23) 30 dpf larvae raised under a non-calorie restricted SD. Data points are the body lengths of individual 30 dpf larvae that survived until the end of the feeding experiments.

(D) Quantitation of mutant/heterozygous control body length ratios for cubn ^pd1169 and dab2 ^pd1162 larvae before onset of feeding (5 dpf) and after being fed with a non-calorie restricted SD (30 dpf). Growth deficits largely occur post-embryonically.

(E) Intestine cross section images of 10 dpf dab2 ^pd1162 +/− or −⁄− larvae raised under non-calorie restricted SD. Arrowheads point to 4E8 colocalization with phallodin, indicating no polarization defect in some of the dab2 −⁄−. L = lumen. n ≥ 15 animals/genotype. Scale bar, 20 μm. ^∗p < 0.05, ^∗∗∗∗p < 0.0001; two-tailed unpaired t tests (A–C). p values, t values and degree of freedom for the statistical tests are provided in the Table S3.