Fig. 3.

Immune cell motility and directionality within suture-adjacent tissues are affected by implanted material. (A) Endpoints from 180 min long representative time lapse movies of Tg(mpeg:mCherry) transgenic adult zebrafish at the indicated time points of uninjured, post-pull through (PT, white circle) or suture implant (red and red braided circles for nylon and vicryl, respectively), showing the tracks of macrophages as they respond to the wound/suture. Tracks are generated by automated cell tracking software (see Materials and Methods) and are indicated by white lines; the area of wounding or implantation is marked by a white asterisk; the dotted lines indicates nylon suture; boxed areas shown in lower row. n=4 independent fish per condition, per tim epoint. (B) Quantification of mean±s.d. macrophage speed at 1 day and 28 days post implantation, averaged from tracking data, indicating how motility is suppressed at later time points, particularly with vicryl sutures. Statistical significance, as measured by one-way ANOVA, is P=0.0045. (C) Quantification of directionality of macrophages at 1 and 28 days post implantation, averaged from tracking data. Statistical significance, as measured by one-way ANOVA, is P=0.0078. *P≤0.05; **P≤0.001; NS, not significant. Scale bars: 200 μm.