FIGURE

Fig. 2.

ID
ZDB-FIG-191230-1890
Publication
Liu et al., 2019 - Combinatorial genetic replenishments in myocardial and outflow tract tissues restore heart function in tnnt2 mutant zebrafish
Other Figures
All Figure Page
Back to All Figure Page
Fig. 2.

Myocardial-specific and DOX-inducible tnnt2a expression fail to rescue heart function in tnnt2a mutant zebrafish. (A) Structure of the tol2-flanked transgenic vector. DOX, doxytetracycline. (B) Lateral views of a 72 hpf transgenic zebrafish after DOX induction at 48 hpf in green and red fluorescence. Scale bar: 300 µm. (C) Real-time RT-PCR of wild-type form of tnnt2a cDNA in wild-type, tnnt2a−/−, Tg(myl7:tetOn; tre:tnnt2a-p2a-mKate2; myl7:eGFP) [Tg(myl7:tnnt2a)] following addition of DOX at 16 hpf, and Tg(myl7:tetOn; tre:tnnt2a-p2A-mKate2; myl7:eGFP; tnnt2a−/−) [Tg(myl7:tnnt2a); tnnt2a−/−] following addition of DOX at 16 or 48 hpf. **P<0.01, ***P<0.001, ****P<0.0001, NS, not significant; n=30 per group. Statistical differences were evaluated using two-way ANOVA. (D) Representative images of hearts of four groups from ventral view. Yellow dotted line, pericardium; white line, heart outline; black box, area of the fluorescent images; V, ventricle; A, atrium. Scale bar: (bright field) 50 µm, (fluorescent) 100 µm. DOX was added at 16 hpf. (E–H) Statistics for heart rate, atrial and ventricular end-diastolic diameter, and pericardium size in four groups; **P<0.01, ****P<0.0001, NS, not significant; n=15 per group. Statistical differences were evaluated using Kruskal–Wallis test (E) or one-way ANOVA (F–H).

Expression Data
Genes:
Fish:
Condition:
Anatomical Term:
Stage: Protruding-mouth

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Condition:
Observed In:
Stage: Protruding-mouth

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Biol. Open