FIGURE

Fig 5

ID

ZDB-FIG-191230-1316

Publication

Chen et al., 2019 - A missense mutation in SNRPE linked to non-syndromal microcephaly interferes with U snRNP assembly and pre-mRNA splicing

Other Figures

All Figure Page

Back to All Figure Page

Fig 5

The SNPRE/SmE deficiency interferes with zebrafish brain development.

(A), Measurement of zebrafish head size across different experimental conditions. CO-MO, control morpholino; E-MO, SmE morpholino. The morpholino and/or in vitro transcribed RNA are injected into embryo at 1-cell stage. Yellow line marked the region for quantification. (B), Quantification of zebrafish head size. Left, head size; middle, body length; right, head size normalized by body length. UN, un-injected; CO-MO, control morpholino; E-MO, SmE morpholino; WT, Wide type SmE gene in vitro transcript; Mut, mutant SmE gene in vitro transcript; The UN is normalized to 1. * P<0.05; ** P<0.01. (C), PCA analysis of the expression of 16067 protein coding genes (RPKM > 1) in zebrafish head and tail samples under different conditions. (D), Numbers of differentially expressed genes comparing to CO-MO. (E), Proportion of DEGs in 14 significant enriched dysregulated GO terms (biologic process, E-MO versus CO-MO, BH-adjusted p < 0.001). (F), Overlap of enriched dysregulated KEGG pathways (E-MO versus CO-MO, BH-adjusted p < 0.001) between zebrafish head and tail samples. G, Numbers of aberrant splicing events comparing to CO-MO.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	TL + MO1-snrpe
Knockdown Reagent:	MO1-snrpe
Observed In:	head swim bladder whole organism
Stage:	Long-pec

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.