FIGURE 8

Mitochondrial quality is indispensable for embryogenesis. (A) Live embryos at 48 hpf after administration of FCCP with different doses and exposure durations. (B) Spontaneous locomotion in 5 dpf larvae after FCCP with different doses and durations. Slow velocity green lines are speeds between 3 and 6 mm/s. Fast velocity red lines are speeds > 6 mm/s (n = 12 fish per group). (C) Confocal imaging of mitochondrial network (Tomm20, T20; green) counterstained with Phalloidin (Ph; red), Dystrophin (Dys; magenta) and Hoescht (H; blue) with or without FCCP administration (500 nM from 24 to 28 hpf). (D,E) Basal respiration (D) and maximal respiration (E) in 48 hpf embryos with or without FCCP administration (500 nM from 24 to 28 hpf, n = 10 fish per group). Quantitative data are mean ± SEM. ^∗P < 0.05, ^∗∗∗P < 0.0001, unpaired T-test compared to control embryos (Ctrl). See also Supplementary Figure S8 and Supplementary Videos S2, S3.