FIGURE 2

Mitochondria network adaptation follows three patterns of change through embryogenesis. (A) Confocal imaging of mitochondrial network (Tomm20, T20; green) counterstained with Phalloidin (Ph; red), Dystrophin (Dys; Magenta) and Hoescht (H; blue) at 18, 20, 24, 28, 36, and 48 hpf. Pictures taken at the limit of yolk extension (somites 15–17). (B) Electron micrographs of longitudinal sections at 20, 24, 28, and 48 hpf. Green overlays highlight mitochondria, red arrows indicate sarcomeric structures, magenta line is the separation between two somites. (C) Quantitative analyses of mitochondrial number, area and circularity at 20, 24, 28, and 48 hpf (n = 6 micrograph areas of 200 × 200 μm² analyzed per group). (D) Quantification of Tomm20-zsGreen fluorescence ratio between somite center and boundary region at 20, 24, 28, and 48 hpf (n = 6 fish per group, 3 images analyzed per fish). (E) Cartoon depicts three distinct patterns presented by the mitochondrial network through embryogenesis. Bars are mean ± SEM. ^∗P < 0.05, ^∗∗P < 0.01, ^∗∗∗P < 0.001, one way repeated measures ANOVA with Tukey HSD post hoc test. See also Supplementary Figures S2–S5 and Supplementary Video S1.