Behavior of vldlr–/– zebrafish. (A,B) Social preference test. (A) Both genotypes prefer to spend time near a group of unfamiliar fish [1st strangers; p < 0.0001 for both genotypes; two-way ANOVA followed by Sidak’s post hoc, genotype factor: F(1,24) = 0.0039, p = 0.95, stranger factor: F(1,24) = 120.1, p < 0.0001, interaction genotype × stranger: F(1,24) = 0.4912, p = 0.49]. (B) WT and vldlr–/– spend equal time near both groups when a second unfamiliar group is added [p = 0.58 for WT and p = 0.73 for vldlr–/–; two-way ANOVA followed by Sidak’s post hoc, genotype factor: F(1,24) = 1.353, p = 0.26, stranger factor: F(1,24) = 0.005, p = 0.94, interaction genotype × stranger: F(1,24) = 0.02779, p = 0.87. n = 8 wild-type and n = 7 vldlr–/–]. (C–E)vldlr–/– behavior in the open field test. (C)vldlr–/– spent the same time at side of the tank [t-test (Welch): t(1.581) = 9.068, p = 0.15] compared to WT but more (D) time in center of the tank [t-test (Welch): t(2.595) = 9.47, p = 0.03]. (E) Locomotion [t-test (Welch): t(0.3779) = 12.84, p = 0.71. n = 8 wild-type and n = 7 vldlr–/–]. (F)vldlr–/– exhibit normal anxiety-like behavior. Time at the bottom of novel tank (Mann Whitney test: U = 16, p = 0.32), (G) locomotion in a novel tank [t-test (Welch): t(0.1803) = 10.92, p = 0.86. n = 7 wild-type and n = 7 vldlr–/–]. (H) No difference in aggression levels between WT and vldlr–/– [t-test (Welch): t(1.87) = 11.41, p = 0.09. n = 8 WT and n = 7 vldlr–/–]. ∗p < 0.05, ****p < 0.0001.
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