Fig. 3

Loss of adhesion molecules results in aberrant F-Actin distribution along the myelin sheath in zebrafish.  a Myelin sheaths in 3 dpf wild-type (wt) and  cntn1b^−/− mag^−/− fish expressing Lifeact-tagRFP to label F-actin. Lifeact-tagRFP displayed a line pattern distribution in both wt and mutant.  b Myelin sheaths in 4 dpf wt and  cntn1b^−/− mag^−/− fish expressing Lifeact-tagRFP. Lifeact-tagRFP displayed a line pattern distribution in wt and a barbell pattern in mutants.  cRepresentative images and quantification of Lifeact-tagRFP fluorescence intensities along example sheaths at 4 dpf.  dRatio of barbell-shaped and line-shaped patterns in myelin sheaths ( n = 3–5) at 3 and 4 dpf.  e Myelin sheaths in7 dpf wt and  cntn1b^−/− mag^−/− fish expressing Lifeact-tagRFP. Lifeact-tagRFP had almost disappeared in wt, whereas the barbell pattern persisted in mutants.  f Quantification of the area occupied by F-actin within myelin sheaths at 7 dpf in wt and  cntn1b^−/− mag^−/− fish ( n = 11–14). Images ( a, b, e) are maximum intensity projections of Tg(mbp:EGFP-CAAX) zebrafish dorsal spinal cord.  p values, *** < 0.001. Data are presented as means ± s.d. Scale bars, 10 μm ( a, b, e), 2 μm ( c). Source data are provided as a Source Data file