Fig. S6

Myotendinous junctions and muscle of scxakg141 and scxb mutants appear normal. A-A’. Small confocal stacks of immunofluorescence for MyHC (A4.1025, green), Tsp4b (red) and nuclear stain (Hoechst) of 4 dpf wt embryos somites 11,12, in lateral view, showing cells (magnified in A’) at the edge of myosepta (white arrowheads) surrounded by the somites and the neural tube (nt), in which single nuclei (yellow arrowheads) are surrounded by Tsp4b matrix stain and extend processes of Tsp4b along the myosepta (arrows). m, muscle fibres. B,C. Lateral view of somites 11- 14 of confocal stacks for immunofluorescence of MyHC (A4.1025, green), Tsp4b (red) of 6 dpf embryos from a scxakg141/+ incross (B) or 4 dpf of a scxb+/- incross (C). No major differences in either MyHC or Tsp4b are detected. D. Lateral view of confocal stacks for GFP, Laminin and sarcomeric alpha-actinin of somites 11-14 from 2 dpf scxakg141/+;Tg(actc1b:egfp)zf13 incross embryos. No major differences between genotypes are detected. All scale bars 50μm, except A-A’, 20μm.