FIGURE

Fig. 7

ID

ZDB-FIG-190806-23

Publication

Baumgartner et al., 2019 - Identification of regulatory elements recapitulating early expression of L-plastin in the zebrafish enveloping layer and embryonic periderm

Other Figures

All Figure Page

Back to All Figure Page

Fig. 7

In vivo validation of zebrafish first intron promoter and enhancer activity.

A. LEFT: 5′ deletion series of the zebrafish 5′ homology arm upstream of the transgenic cassette (lcp1:Cre-P2A-EGFP-CAAX, abbreviated ‘EGFP’). Constructs are numbered according to the number of bases upstream of the engineered translation start site (ATG, where A = +1.) A. RIGHT: Results of deletion-series injections. Green bars = proportion of injected embryos with EGFP ⁺ EVL cells. Gray lines = 95% confidence interval on the proportion.

B-F. Animal cap views of injected, EGFP ⁺ embryos collected at 50% epiboly.

G. Annotation of the minimal intronic region (−481 to −169) sufficient for EVL activity. The deletion-construct forward PCR primers are underlined. The predicted transcript is in UPPER CASE. TATA = TATA box motif; TSS = transcription start site; DPE = downstream promoter element. The EST ‘foothill’ (See Fig. 7, Fig. 8) is shaded yellow.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Gene expression patterns : GEP, 32, Baumgartner, E.A., Compton, Z.J., Evans, S., Topczewski, J., LeClair, E.E., Identification of regulatory elements recapitulating early expression of L-plastin in the zebrafish enveloping layer and embryonic periderm, 53-66, Copyright (2019) with permission from Elsevier. Full text @ Gene Expr. Patterns