ZFIN Image: Baumgartner et al., 2019, Fig. 7

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Figure Caption

Fig. 7

In vivo validation of zebrafish first intron promoter and enhancer activity.

A. LEFT: 5′ deletion series of the zebrafish 5′ homology arm upstream of the transgenic cassette (lcp1:Cre-P2A-EGFP-CAAX, abbreviated ‘EGFP’). Constructs are numbered according to the number of bases upstream of the engineered translation start site (ATG, where A = +1.) A. RIGHT: Results of deletion-series injections. Green bars = proportion of injected embryos with EGFP ⁺ EVL cells. Gray lines = 95% confidence interval on the proportion.

B-F. Animal cap views of injected, EGFP ⁺ embryos collected at 50% epiboly.

G. Annotation of the minimal intronic region (−481 to −169) sufficient for EVL activity. The deletion-construct forward PCR primers are underlined. The predicted transcript is in UPPER CASE. TATA = TATA box motif; TSS = transcription start site; DPE = downstream promoter element. The EST ‘foothill’ (See Fig. 7, Fig. 8) is shaded yellow.

Acknowledgments

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Reprinted from Gene expression patterns : GEP, 32, Baumgartner, E.A., Compton, Z.J., Evans, S., Topczewski, J., LeClair, E.E., Identification of regulatory elements recapitulating early expression of L-plastin in the zebrafish enveloping layer and embryonic periderm, 53-66, Copyright (2019) with permission from Elsevier. Full text @ Gene Expr. Patterns