Fig. 5

The Crosstalk between CHT Niche and HSPCs

(A) SOM showing the distinct gene regulatory structure among NCs, ECs, and HSPCs at 52 hpf and 3 and 4 dpf.

(B) Secretomics analysis showing the ligand-receptor regulatory relationship between HSPCs and CHT niche at 52 hpf.

(C) The secretomics analysis for scRNA-seq data revealing the interaction pairs in distinct cell types, receptor-expressing C4 (proliferating HSPC) and ligand-expressing clusters.

(D) The distribution of ligands and receptors in ECs (kdrl⁺ cells), HSPCs (CD41⁺cells), and NCs (kdrl^-CD41^- cells) at 52 hpf and 3 dpf based on bulk RNA-seqanalysis.

(E) Double FISH showing the co-expression of ctgfa with fli1a, and itgb2 with cmyb at 2 dpf. Scale bar, 50 μm.

(F) The confocal imaging of the transgenic line (kdrl:mCherry/runx1:en-GFP) showing the number of runx1⁺ cells in control, ctgfa morphants, and itgb2morphants at 4 dpf. Scale bar, 50 μm.

(G) The quantification of confocal imaging displayed in (F) are shown as means ± SEM. ^∗p < 0.05, n = 8. The HSPCs were counted in about the eight-somite region of the CHT in control embryos and morphants.