FIGURE

Fig. 4

ID

ZDB-FIG-190723-85

Publication

Yang et al., 2019 - Cul4a promotes zebrafish primitive erythropoiesis via upregulating scl and gata1 expression

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Fig. 4

The expression of the early hematopoietic markers <italic>scl</italic> and <italic>lmo2</italic> was downregulated in <italic>cul4a</italic> mutants.

a, b WISH was performed with scl (a) or lmo2 (b) probes in embryos at 6 somite and 24 hpf, respectively. Embryos at 6-somite stage were in poster order view with anterior to the top. The ICM regions of embryos were lateral views with anterior to the left. c, d qRT-PCR analyzed the expression of scl and lmo2 in embryos injected with CoMO or cul4a-MO, or MO coinjected with zebrafish cul4a mRNA at 6 somite and 24 hpf. e, f The images of WISH with scl or lmo2 mRNA probes in control (cas9-tail injected), cul4a^−/−, cul4b^−/−, and double knockout mutants at 24 hpf. g, h Relative expression of scl and lmo2 mRNA were analyzed by qRT-PCR in control and cul4a^−/−, cul4b^−/−, and double knockout mutants. The number in the top right-hand corner indicates the phenotypic embryos/total embryos. qRT-PCR experiments were performed in triplicate. ***p < 0.001. All scale bars represent 250 μm

Expression Data

Genes:	lmo2 tal1
Fish:	WT cul4a^{zf3203/zf3203} cul4b^{zf3204/zf3204} WT + MO2-cul4a cul4a^{zf3203/zf3203}; cul4b^{zf3204/zf3204}
Knockdown Reagent:	MO2-cul4a
Anatomical Term:	hematopoietic cell
Stage Range:	5-9 somites to Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	cul4a^{zf3203/zf3203} cul4b^{zf3204/zf3204} WT + MO2-cul4a cul4a^{zf3203/zf3203}; cul4b^{zf3204/zf3204}
Knockdown Reagent:	MO2-cul4a
Observed In:	hematopoietic cell
Stage Range:	5-9 somites to Prim-5

Phenotype Detail

Acknowledgments

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