IMAGE

Fig. 4

ID
ZDB-IMAGE-190723-126
Genes
Source
Figures for Yang et al., 2019
Image
Figure Caption

Fig. 4 The expression of the early hematopoietic markers <italic>scl</italic> and <italic>lmo2</italic> was downregulated in <italic>cul4a</italic> mutants.

a, b WISH was performed with scl (a) or lmo2 (b) probes in embryos at 6 somite and 24 hpf, respectively. Embryos at 6-somite stage were in poster order view with anterior to the top. The ICM regions of embryos were lateral views with anterior to the left. c, d qRT-PCR analyzed the expression of scl and lmo2 in embryos injected with CoMO or cul4a-MO, or MO coinjected with zebrafish cul4a mRNA at 6 somite and 24 hpf. e, f The images of WISH with scl or lmo2 mRNA probes in control (cas9-tail injected), cul4a−/−, cul4b−/−, and double knockout mutants at 24 hpf. g, h Relative expression of scl and lmo2 mRNA were analyzed by qRT-PCR in control and cul4a−/−, cul4b−/−, and double knockout mutants. The number in the top right-hand corner indicates the phenotypic embryos/total embryos. qRT-PCR experiments were performed in triplicate. ***p < 0.001. All scale bars represent 250 μm

Figure Data
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Cell Death Dis.