FIGURE

Fig. 5.

ID

ZDB-FIG-190723-2562

Publication

Skvarca et al., 2019 - Enhancing acute kidney injury regeneration by promoting cellular dedifferentiation in zebrafish

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Fig. 5.

UPHD25 treatment has no effect on neutrophil response but lowers total macrophage recruitment during early AKI phase. (A,C) Tg(cdh17:mCherry); Tg(lyz:EGFP) transgenic zebrafish were used for neutrophil analyses. (B,D) Tg(cdh17:mCherry); Tg(mpeg1;dendra2) transgenic zebrafish were used for macrophage analyses. (A,B) Immunofluorescence co-stain of PTs (red or green) and neutrophils (green) (A) and macrophages (red) (B) in gent-AKI+DMSO and gent-AKI +UPHD25. PTs are outlined in white and leukocytes are marked with arrows. (C) Quantification of neutrophil numbers showed no significance between DMSO and UPHD25 treatment groups. (D) Quantification of macrophage numbers showed a significant decrease in macrophage numbers in UPHD25 treatment group. Mean_{Gent-AKI+DMSO}=16.38 (N=69) vs Mean_{Gent-AKI+UPHD25}=12.99 (N=75). Data pooled from three biological replicates are shown expressed as mean±s.e.m. Two-tailed t-test: *P<0.05, ns, not significant. Scale bars: 20 μm.

Expression Data

Genes:	Dendra2 EGFP
Antibody:	Ab-3G8
Fish:	nz117Tg nz4Tg; uwm12Tg
Conditions:	chemical treatment by injection: gentamycin chemical treatment by environment: EC 3.5.1.98 (histone deacetylase) inhibitor, chemical treatment by injection: gentamycin
Anatomical Terms:	macrophage neutrophil pronephric duct regenerating tissue pronephric duct
Stage:	Day 6

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	nz117Tg nz4Tg; uwm12Tg
Conditions:	chemical treatment by injection: gentamycin chemical treatment by environment: EC 3.5.1.98 (histone deacetylase) inhibitor, chemical treatment by injection: gentamycin
Observed In:	macrophage activation involved in immune response neutrophil activation involved in immune response pronephric duct macrophage pronephric duct neutrophil regenerating tissue macrophage regenerating tissue neutrophil
Stage:	Day 6

Phenotype Detail

Acknowledgments

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