FIGURE

Fig. 4.

ID

ZDB-FIG-190723-2561

Publication

Skvarca et al., 2019 - Enhancing acute kidney injury regeneration by promoting cellular dedifferentiation in zebrafish

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Fig. 4.

Neutrophil and macrophage populations change in the kidney field after AKI. (A-D) Tg(cdh17:mCherry); Tg(lyz:EGFP) transgenic zebrafish were used for neutrophil analyses. (E-H) Tg(cdh17:mCherry); Tg(mpeg1;dendra2) transgenic zebrafish were used for macrophage analyses. (A,E) Transgenic lines were injected with gentamicin at 3 dpf and imaged at 2 dpi. (B,F) Snapshots of live imaging of lyz+ neutrophils imaged at 1 dpi for 13.5 h (B) and mpeg1+ macrophages imaged at 2 dpi for 19.5 h (F) in a no-injury and gent-AKI setting. Arrows indicate neutrophils or macrophages adjacent to PTs. (C,G) Immunofluorescence co-stain of PTs (red or green) and neutrophils (green) (C) or macrophages (red) (G) in no-injury and gent-AKI at 3 dpi. PTs are outlined in white and adjacent leukocytes are marked with arrows. (D,H) Quantification of neutrophil (D) and macrophage (H) numbers adjacent to the PT before and after injury. Mean_NoInjury5dpf=4.24 (N=34) vs Mean_2dpi=7.76 (N=34) vs Mean_NoInjury6dpf=5.15 (N=34) vs Mean_3dpi=11.60 (N=48). Adjacent leukocytes were counted for both no injury and gent-AKI at 1, 2 and 3 dpi (D), and 2 and 3 dpi (H). Data pooled from three biological replicates are shown expressed as mean±s.e.m. One-way ANOVA: *P<0.05, **P<0.01, ***P<0.005. Scale bars: 20 μm.

Expression Data

Genes:	Dendra2 EGFP mCherry
Antibody:	Ab-3G8
Fish:	nz117Tg nz4Tg; uwm12Tg nz117Tg; pt307Tg pt307Tg; uwm12Tg
Condition:	chemical treatment by injection: gentamycin
Anatomical Terms:	macrophage neutrophil pronephric duct pronephric tubule regenerating tissue pronephric duct regenerating tissue pronephric tubule
Stage Range:	Pec-fin to Day 6

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	nz117Tg nz4Tg; uwm12Tg nz117Tg; pt307Tg pt307Tg; uwm12Tg
Condition:	chemical treatment by injection: gentamycin
Observed In:	macrophage activation involved in immune response neutrophil activation involved in immune response pronephric duct macrophage pronephric duct neutrophil regenerating tissue macrophage regenerating tissue neutrophil
Stage Range:	Pec-fin to Day 6

Phenotype Detail

Acknowledgments

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