Investigation of mpv17 orthologue and paralogue genes in zebrafish larvae. (A) Real-time PCR quantification of mRNA transcripts of mpv17-like2 and mpv17-like at 6 dpf (n=9). (B) Evaluation of phenotypic rescue in the tail region at 3 dpf after the injection of human MPV17mRNAs, wild-type (WT) and p.R50Q mutated forms, and zebrafish mpv17 and mpv17-like2mRNAs. Arrowheads point to iridophores. Scale bars: 100 µm. (C) Relative quantification of iridophore amount in controls and injected larvae (n=30). (D) mtDNA copy number analysis in mpv17−/− mutants transiently overexpressing mpv17-like2 at 3 dpf and 6 dpf. Mean dCt values were calculated as Ct of mt-nd1 (mitochondrially encoded gene) minus Ct of polg (nuclear gene) (n=4). Statistical analyses were performed using two-tailed Student's t-test. Statistical significance was evaluated by setting a confidence interval of 95%; data are mean±s.e.m. ****P<0.0001; ***P<0.001; *P<0.05; ns, not significant. Experiments were performed in biological replicates.
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