Figure 5

Co-injection of cpsf1 MO and p53 MO in zebrafish failed to rescue the small eye size phenotype. (A) Eye size in zebrafish at 3 dpf. The eye diameter (eye size) was 293.83 ± 10.17 μm (n = 67) for the std control, 261.75 ± 21.04 μm (n = 51) for the cpsf1 morphants, and 261.86 ± 19.67 μm (n = 55) for the zebrafish subjected to cpsf1-MO/p53 MO co-injection. Small eye size was detected in cpsf1 morphants, but this phenotype could not be rescued by p53 MO. Std MO-injected zebrafish were used as a control in this study. (B) Quantification of small eye size proportions in zebrafish at 3 dpf. The proportion of small eye size was significantly increased in cpsf1 morphants compared to that of the std control (P = 6.06 × 10⁻¹⁴), but it was still increased compared to that in the std control after co-injection of cpsf1 MO and p53 MO (P = 7.90 × 10⁻¹⁵) based on the Chi-square test (P < 0.017, or 0.05/3, was considered as statistically significant). The number of zebrafish injected in each group is indicated under each column.