FIGURE

Fig. 4

ID

ZDB-FIG-190709-2

Publication

Ahsan et al., 2019 - Prickle1 is required for EMT and migration of zebrafish cranial neural crest

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Fig. 4

pk1b and pk1a mutants do not show alterations in pharyngeal cartilage fates. (A-C) In situ hybridizations for the pharyngeal neural crestmarker dlx2 in WT (A), pk1b ^{fh122/ fh122} (B) and pk1a^ch105/ch105 (C) 24 hpf embryos in lateral view show no significant differences between conditions. Solid arrowheads indicate streams of NCCs. (D-F) Alcian Green labeling of cartilage elements of the pharyngeal apparatus in WT (D), pk1b ^{fh122/ fh122} (E) and pk1a^ch105/ch105 (F) 6 dpf larvae, show no significant changes in size and organization of elements between conditions. m=Meckel’s cartilage; pq=palatoquadrate; ch=ceratohyal; cb=ceratobranchial; cb(i) indicates first branchial arch, cb(iv) indicates fourth branchial arch, with solid arrows indicating the corresponding ceratobranchial cartilage elements.

Expression Data

Gene:	dlx2a
Fish:	WT prickle1a^ch105/ch105 prickle1b^fh122/fh122
Anatomical Terms:	neural crest pharyngeal arch migratory neural crest
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	prickle1a^ch105/ch105 prickle1b^fh122/fh122
Observed In:	splanchnocranium
Stage:	Day 6

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 448(1), Ahsan, K., Singh, N., Rocha, M., Huang, C., Prince, V.E., Prickle1 is required for EMT and migration of zebrafish cranial neural crest, 16-35, Copyright (2019) with permission from Elsevier. Full text @ Dev. Biol.