Fig. 1

Characterization of zebrafish muscle wasting in kras^G12V-induced HCC zebrafish.4-month-old male adult kras^G12V and WT zebrafish were treated with dox for 4 weeks and sampled at 0 wpi, 2 wpi and 4 wpi. In each group, 15 fish were used to initiate the experiments. (A) Gross appearance and liver morphology (left), body weight excluding internal viscera (middle) and survival curves (right). (B) H&E staining of liver sections of kras^G12V fish. Quantification of tumor histology (right). (C) IF staining of PCNA (red), Hnf4a (green) and DAPI (blue) in liver sections of kras^G12V fish. Quantification of percentage of proliferating hepatocytes (right). (D) H&E staining of muscle sections of kras^G12V fish. Quantification of MFCSA (right). (E) Gomori's trichrome staining of muscle sections of kras^G12V fish. Quantification of percentage of collagen deposited area (right). (F) Correlation between percentage of proliferating cells in the liver (x-axis) and MFCSA (y-axis) at 0 wpi (left), 2 wpi (middle) and 4 wpi (right). *P<0.05. Scale bars: 2.5 mm in A; 10 μm in B-E.