Fig. S5

Statin treatment during LR asymmetry determination is sufficient to produce ciliopathy defects a, Zebrafish embryos were treated from tailbud stage until the 20 somites stage (ss) with atorvastatin or mevastatin. After washout of the drugs embryos were incubated until the 48 hpf stage. b, Live images of 48 hpf embryos treated either with vehicle (1% DMSO in egg water, Control), 10 or 50 μM atorvastatin or 20 nM mevastatin. Arrows indicate pericardiac oedema and curved tails. Scale bar: 200 μm. c, Live images of pericardiac oedema in higher magnification. Scale bar: 200 μm. d, The formation of pericardiac oedema increases with atorvastatin and mevastatin treatment. n=6 (DMSO, 10 μM atorv., 20 nM mevastatin) and 4 (50 μM atorv.) experiments. ** means p<0.01, *** indicates p<0.001. One-way ANOVA with Bonferroni's multiple comparisons test. e, Heart rate is also decreased when statins are only administered during left-right (LR) asymmetry cilia stages. **** p<0.0001, Kruskal-Wallis test with Dunn’s multiple comparison test. f, Mevastatin produces curved bodies. n=6 (DMSO, 10 μM atorv., 20 nM mevastatin) and 4 (50 μM atorv.) experiments. ** indicates p<0.001, Kruskal-Wallis test with Dunn’s multiple comparison test. d - f, circles: individual embryos (e) or experiments (d, f), red line: median. 7 Number of embryos given below graphs.