Fig. 1

Knocking down znfl1s disturbs the development of posterior but not anterior part of zebrafish hindbrain. Flat-mount embryos at 11–12-somites stage are shown anterior left (A–C, F–H, K–M, P–R). The expression of en2a marks the hindbrain-mindbrian boundary (A–C, F–H, K–M, P–R). The expression of egr2b marks r3/r5 territory (A–C, F–H). The expression of mafba marks the r5/r6 (K–M, P–R). The expression of myod1 marks somite (A–C, F–H, K–M, P–R). At 11–12-somite stage, the lengths of r1–r4, r6–s1 and r7–s1 were measured from control embryos (A, F, K, P), znfl1s morphants (B, L), znfl1s MO plus znfl1 mRNA overexpressed embryos (C, M), dCas9-Eve knockdown embryos (G, Q) and dCas9-Eve plus znfl1 mRNA overexpressed embryos (H, R), respectively. The data about the lengths of r1–r4, r6–s1 or r7–s1 derived from A–C, F–H or K–M, P–R are shown in scatter plot diagrams D–E, I–J, N–O and S–T, respectively. The red line in A–C, F–H or in K–M, P–R shows the length of r6–s1 or r7–s1, respectively. The black line in A–C, F–H or in K–M, P–R shows the length of r1–r4. r, rhombmere; s1, the first somite. **: p < 0.01, ns: no significance.