Fig. 4

(A) Growth kinetics of the eye in wildtype (blue line) and Ztcf7l1a^-/- (red line) embryos at stages indicated (data in Supplementary file 1F, one experiment, 24hpf, wt n = 12, Ztcf7l1a^-/-n = 14; 28hpf, wt n = 15, Ztcf7l1a^-/- n = 12; 32hpf, wt n = 13, Ztcf7l1a^-/- n = 15; 36hpf, wt n = 16, Ztcf7l1a^-/- n = 14; 48hpf, wt n = 11, Ztcf7l1a^-/- n = 19; 60hpf, wt n = 11, Ztcf7l1a^-/- n = 14; 72hpf, wt n = 13, Ztcf7l1a^-/- n = 19; 96hpf, wt n = 13, Ztcf7l1a^-/- n = 15). (B) Plot showing the ratio of Ztcf7l1a^-/- to wildtype eye volume from data in (A). (C–L) Lateral views (dorsal up, anterior to left) of wildtype (C–G) and Ztcf7l1a^-/- (H–L) eyes at stages indicated above panels. (M–O) Eye development following partial ablation of the optic vesicle in wildtype embryos at five somite stage. (M) Coronal confocal section of evaginating optic vesicles (red) in a wildtype Tg(rx3:RFP) five somite stage embryo. Dashed line indicates the approximate extent of ablations performed. 36hpf (N) and 4dpf (O) eyes in embryos in which cells were unilaterally removed from one optic vesicle (from n = 20). Asterisk indicates the eye that develops from the partially ablated optic vesicle. ZO1, zona ocludens 1. Scale bars = 200 µm.