Fig. S3

Homozygous itln3uta145/uta145 and itln3uta148/uta148 mutants develop normally. A) F3-progeny of itln3uta145/+ and itln3uta148/+ zebrafish were imaged at 1, 4 and 7 dpf. The larvae were anesthetized for imaging with 0.02% 3-amino benzoic acid ethyl ester (Sigma-Aldrich) at 4 and 7 dpf, and the embryos collected for genotyping at 7 dpf. Representative images of itln3uta145/145 and itln3uta148/148 mutants as well as the corresponding WT embryos are shown. Micrographs were taken with Zeiss Lumar V12 fluorescence microscope and Axiocam MRm digital camera using a bright field exposure of 2 ms. A 23.5x-magnification was used at 1 dpf and a 15.0x-magnification at 4 and 7 dpf. B) 12-month-old WT (itln3uta145), itln3uta145/145 , WT (itln3uta148) and itln3uta148/148 female and male zebrafish were anesthetized with 0.02% 3-amino benzoic acid ethyl ester (Sigma-Aldrich) and imaged submerged in water using Canon EOS 7D Mark II camera with an exposure time of 8 ms. Brightness was increased by 20% for all of the images in panel B using Windows Photo Viewer. All images in panels A-B were cropped to exclude unnecessary empty background from the figure.