ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions.

ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions.

ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions.

ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions.

ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions.

Dexamethasone mediated immunosuppression does not alter the survival of itln3 deficient zebrafish in a mycobacterial infection. (A) A schematic representation of the performed experiment. M. marinum inoculate used in the infections were 47 CFU (SD 4 CFU). (B) Representative flow cytometry plots in WT (itln3^uta148) zebrafish at -1 wpi and 2 wpi used for quantifying lymphocyte, myeloid cell and precursor cell populations. FSC = forward scatter, SSC = side scatter. (C,D) Lymphocyte fractions of the total cell populations for both WT and itln3 knockout zebrafish at -1 wpi and 2 wpi. Both the itln3^uta145 (n = 12 in all groups) and itln3^uta148 background fish (n = 8 in all groups) are shown. Blood cell samples were run as technical duplicates. (E,F) M. marinum burden (log10) in 100 ng of zebrafish DNA were measured at 2 wpi and 4 wpi by qPCR in the infected dexamethasone treated zebrafish organ blocks (without the kidney). All bacterial quantification samples were run once. Group sizes at 2 and 4 wpi, respectively, were as follows: WT (itln3^uta145) n = 9, n = 8; iltn3^{uta145/uta145}n = 9, n = 10; WT (itln3^uta148) n = 6, n = 9 and iltn3^{uta148/uta148} n = 7, n = 8. A two-tailed Mann-Whitney test was used in the statistical comparison of differences.

Homozygous itln3uta145/uta145 and itln3uta148/uta148 mutants develop normally. A) F3-progeny of itln3uta145/+ and itln3uta148/+ zebrafish were imaged at 1, 4 and 7 dpf. The larvae were anesthetized for imaging with 0.02% 3-amino benzoic acid ethyl ester (Sigma-Aldrich) at 4 and 7 dpf, and the embryos collected for genotyping at 7 dpf. Representative images of itln3uta145/145 and itln3uta148/148 mutants as well as the corresponding WT embryos are shown. Micrographs were taken with Zeiss Lumar V12 fluorescence microscope and Axiocam MRm digital camera using a bright field exposure of 2 ms. A 23.5x-magnification was used at 1 dpf and a 15.0x-magnification at 4 and 7 dpf. B) 12-month-old WT (itln3uta145), itln3uta145/145 , WT (itln3uta148) and itln3uta148/148 female and male zebrafish were anesthetized with 0.02% 3-amino benzoic acid ethyl ester (Sigma-Aldrich) and imaged submerged in water using Canon EOS 7D Mark II camera with an exposure time of 8 ms. Brightness was increased by 20% for all of the images in panel B using Windows Photo Viewer. All images in panels A-B were cropped to exclude unnecessary empty background from the figure.