FIGURE

Fig. S13

ID

ZDB-FIG-190611-47

Publication

Neal et al., 2019 - Venous identity requires BMP signalling through ALK3

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Fig. S13

alk3a/b is required for Ephb4-2 expression and is expressed in the early zebrafish axial vein a-b. Directly relating to Fig. 8b. Dose-response analysis of Ephb4-2:GFP expression and morphology using different concentrations of alk3a and alk3b morpholinos. Representative embryos are shown in a and expression patterns summarized in b. 3.5ng alk3aMO/0.9ng alk3bMO embryos n=54 at 36hpf, 51 at 48hpf; 14ng alk3aMO/2.7ng alk3bMO embryos n=32 at 36hpf, n=55 at 48hpf. c. Whole-mount in situ hybridization for alk3a and alk3b expression compared to pan-endothelial kdrl in wild type zebrafish embryos at 10, 15 and 18 somite stage (ss). d. Whole-mount in situ hybridization for ephb4a expression in wild type zebrafish embryos at 15 and 18 and 20 somite stage (ss).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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