Fig. 7

A SMAD1/5-binding peak identifies a vein endothelial enhancer for Coup-TFII. a UCSC browser view of COUP-TFII (NR2F2) incorporating SMAD1/5-binding data from Morikawa et al. A statistically significant SMAD1/5-binding peak in human umbilical vein endothelial cells (HUVECs) (red) −965 kb upstream of Coup-TFII correlated with the H3K27Ac enhancer histone mark in HUVECs (blue peaks) and endothelial HUVEC DNaseI hypersensitivity (black heat map). b, c Stable transgenic mouse (b) and zebrafish (c) embryos expressing the lacZ and GFP reporter genes, respectively, under the control of the murine CoupTFII-965 enhancer. In both animal models, enhancer activity was primarily seen in the venous endothelium. Black and white scale bars both represent 100 μm, grey scale bars are 500 μm, red scale bars are 1000 μm. a = artery, v = vein, cev = branches of cerebral venous plexus, cv = cardinal vein, pm = paraxial mesoderm, da = dorsal aorta, red bracket = dorsal aorta; white bracket = posterior cardinal and ventral vein(s). dTables summarizing reporter gene expression in transient transgenic zebrafish and mouse embryos after mutation of the core SMAD-binding element (SBE-PEAK).