FIGURE

Fig. S1

ID

ZDB-FIG-190611-19

Publication

Delgadillo-Silva et al., 2019 - Modelling pancreatic β-cell inflammation in zebrafish identifies the natural product wedelolactone for human islet protection

Other Figures

All Figure Page

Back to All Figure Page

Fig. S1

Leukocytes express tnfa in Tg(ins:il1b) larvae.

(A-A’) Confocal slices (single plane) showing the primary islets of 3 dpf WT (A) and Tg(ins:il1b) larvae (A’) in the background of a Tg(tnfa:GFP) transcriptional reporter. The arrow in A’ shows an L-plastin-positive cell (magenta), which is also positive for tnfa:GFP (green). This cell is embedded in the islet. Some GFP-positive cells can be observed in the region corresponding to the extra-pancreatic duct in both WT and Tg(ins:il1b) larvae. These cells are L-plastin-negative. (B-C) Quantification of the proportion of GFP-positive cells among the L-plastin-positive cells in contact with the islet at 3 (B), unpaired two-tailed t-test with Welch’s correction, *p-value ≤ 0.05, mean±SD and 5 dpf (C), unpaired two-tailed t-test with Welch’s sorrection, ***p-value ≤ 0.001, mean±SD. (D-E) Quantification of the total number of L-plastin cells in contact with the islet at 3 (D), unpaired two-tailed t-test with Welch’s correction, *p-value ≤ 0.05, mean±SD and 5 dpf (E), unpaired two-tailed t-test with Welch’s correction, ***p-value ≤ 0.001, mean±SD . Scale bars in A-A’= 10 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dis. Model. Mech.