Time-lapse imaging reveals dynamic interactions between β-cells and macrophages under chronic inflammation. (A) Representative snapshots from time-lapse movies from control and Tg(ins:il1b) larvae. Tg(ins:mCherry) labels the β-cells (red), whereas Tg(mpeg1:GAL4);Tg(UAS-Kaede) labels the macrophages (green). β-cells and macrophages were imaged every 5?min for 10?h starting at 4.5?dpf. The elapsed time in hours (hrs) is indicated. (B) Plot showing the time that individual macrophages spend in the islet region as defined using a region of interest (ROI) (n=5 animals each for il1b− and il1b+). In controls (blue), the macrophages rarely visit the islet. In Tg(ins:il1b) larvae (red), the macrophages show an increase in the time they spend in the islet region. Unpaired two-tailed t-test with Welch's correction, ****P<0.0001, mean±s.d.
